@article{oai:repo.qst.go.jp:00044484, author = {Ishii, Nobuyoshi and Koiso, Hiroyuki and Sakashita, Tetsuya and Fuma, Shoichi and Takeda, Hiroshi and Uchida, Shigeo and 石井 伸昌 and 小礒 寛之 and 坂下 哲哉 and 府馬 正一 and 武田 洋 and 内田 滋夫}, issue = {1 / 2}, journal = {International Journal of PIXE}, month = {}, note = {Two types of bleached mutant strains were isolated from a culture of wild-type Euglena gracilis Z strain. These mutant strains formed yellow and white colored colonies, named Y1ZSmL and W1ZSmL, respectively. The losses of chlorophyll a in both mutant strains were found from the evaluation of chlorophyll a concentrations. To elucidate physiological effects due to the chlorophyll a loss, cell growth and the amounts of trace elements (Al, P, S, K, Ca, Fe, Cu, and Zn) in each strain were studied. The obtained data for mutant strains were compared with those for wild-type Z strain. Cell growth of each strain differed and the abundances of cells were consequently high, decreasing in the order of Z, Y1ZSmL, and W1ZSmL. The amounts of all elements except S in mutant strains were significantly lower than those in the Z strain. Differences between Y1ZSmL and W1ZSmL were found only in the amount of Cu. Copper, therefore, would be one of the factors causing differences in the color of the colonies and the growth of cells between both mutant strains.}, pages = {77--83}, title = {Determination of trace elements in Euglena gracilis Z and its bleached mutant strains}, volume = {16}, year = {2006} }