@article{oai:repo.qst.go.jp:00044465,
 author = {Higo, Masahiro and Uzawa, Katsuhiro and Kawata, Tetsuya and Kato, Yoshikuni and Kouzu, Yukinao and Yamamoto, Nobuharu and Shibahara, Takahiko and Mizoe, Junetsu and Ito, Hisao and Tsujii, Hirohiko and et.al and 川田 哲也 and 柴原 孝彦 and 溝江 純悦 and 伊東 久夫 and 辻井 博彦},
 issue = {3},
 journal = {International Journal of Radiation Oncology Biology Physics},
 month = {Jul},
 note = {Purpose:The Purpose of this study was to assess the gene expression changes in oral squamous cell carcinoma (OSCC) cells after carbon ion irradiation.
Methods and Materials: Three OSCC cell lines (HSC2, Ca9-22, and HSC3) were irradiated with accelerated carbon ion beams or X-rays using three different doses.
The cellular sensitivities were determined by clonogenic survival assay.To idenify genes the expression of which is influenced by carbon ion irradiation in a dose-dependent manner. we performed Affymetrix GeneChip analysis with HG-U133 plus 2.0 arrays containing 54,675 probe sets. The idenified genes were analyzed using the Ingenuity Pathway Analusis Tool to investigate the functional network and gene outology. changes in mRNA expression in the genes were assessed by real-time reverse transcriResults: We identified 98 genes with expression levels that were altered sibnificantly at least twofold in each of the three cabon-irradiated OSCC cell lines at all dose points compared with nonirradiated control cells. Among these, SPHK1,the expression of which was significantly upregulated by carbon ion irradiatin, was modulated little by X-rays. The function of SPHK1 related to cellular growth and proliferation had the highest p value (p=9.25e-7 to 2.19e-2).Real-time reverse transcriptase-polymerase chain reaction analysis showed significantly elevated SPHK1 expression leaves after carbon ion irradiation (p < 0.05), consistent with microarray date. Clonogenic survival assay indicated that carbon ion irradiation could induce cell death in Ca0-22 cells more effectively than X-rays.
Conclusions: Our findings suggest that SPHK1 helps to elucidate the molecuar mechanisims and processes underlying the biologic response to carbon ion beams in OSCC.ptase-polymerase chain reaction.},
 pages = {867--875},
 title = {ENHANCEMENT OF SPHK1 IN VITRO BY CARBON ION IRRADIATION IN ORAL SQUAMOUS CELL CARCINOMA},
 volume = {65},
 year = {2006}
}