@article{oai:repo.qst.go.jp:00044417,
 author = {Namba, Hiroki and Fukushi, Kiyoshi and Nagatsuka, Shin-ichiro and Iyo, Masaomi and Shinotoh, Hitoshi and Tanada, Shuji and Irie, Toshiaki and 難波 宏樹 and 福士 清 and 長塚 伸一郎 and 篠遠 仁 and 棚田 修二 and 入江 俊章},
 journal = {Methods},
 month = {},
 note = {A new method for quantitative measurement of brain acetylcholinesterase(AChE) activity in living human brain using positron emission tomography(PET) is described. We tested several radiolabeled lipophilic acetylcholine analogs, e.g., N-methylpiperidyl esters, which readily entered the brain via the blood-brain barrier, were hydrolyzed sselectively by AChE, and were then trapped in the brain. Among them, and tested and N-[11C]methylpiperidin-4-yl acetate ([11C]MP4A) was chosen as the tracer for PET. Quantitative measurement of cortical AChE was accomplished by fitting the time course of cerebral radioactivity concentration measured by PET and the metabolite-corrected arterial plasma input function using a nonlinear least-squares fitting method. Normal control studies with a wide range in age(24-89 years) showed no decrease in AChE activity in the cerebral cortex with age. Studies on patients with Alzheimers disease demonstrated a widespread reduction of AChE activity in the cerebral cortex (more profound in early-onse than in late-onset Alzheimers disease). Parkinsons disease and progressive supranuclear palsy, clinically similar disorders, could be differentiated with [11C]MP4A/PET studies. Simple methods without using an arterial input function are also proposed. The method provides a quantitative measure ot the cholinergic aspect of brain function and proved to be useful in diagnosis of neurodegenerative disorders including Alzheimers disease.},
 pages = {242--250},
 title = {Positron emission tomography: quantitative measurement of brain acetylcholinesterase activity using radiolabeled substrates},
 volume = {27},
 year = {2002}
}