@article{oai:repo.qst.go.jp:00044366,
 author = {Fukumura, Ryuutarou and Araki, Ryoko and Fujimori, Akira and Mori, Masahiko and Saito, Toshiyuki and Watanabe, Fumiaki and Sarashi, Mika and Itsukaichi, Hiromi and Eguchi-Kasai, Kiyomi and Sato, Koki and Tatsumi, Kouichi and Abe, Masumi and 福村 龍太郎 and 荒木 良子 and 藤森 亮 and 森 雅彦 and 齋藤 俊行 and 五日市 ひろみ and 笠井 清美 and 巽 紘一 and 安倍 真澄},
 journal = {The Journal of Biological Chemistry},
 month = {May},
 note = {We established the radiosensitive cell line SX9 from mammary carcinoma cell line FM3A. In SX9 cells a defect of DNA-dependent protein kinase (DNA-PK) activity was suggested. Additionally, a complementation test suggested that the SX9 cell line belongs to a x-ray cross-complementing group (XRCC) 7. Isolation and sequence analyses of DNA-dependent protein kinase catalytic subunit (dna-pkcs) cDNA in SX9 cells disclosed nucleotide "T" (9572) to "C" transition causing substitution of amino acid residue leucine (3191) to proline. Interestingly, the mutation occurs in one allele, and transcripts of the dna-pkcs expressed exclusively from mutated allele. V(D)J recombination assay using extrachromosomal vector revealed the defects of not only coding but also signal joint formation. The frequency of the signal joint decreased to approximately one-tenth and the fidelity drastically decreased to 12. 2% as compared with the normal cell line. To confirm the responsibility of the dna-pkcs gene for abnormal V(D)J recombination in SX9, the full-length dna-pkcs gene was introduced into SX9. As a result, restoration of V(D)J recombination by wild type dna-pkcs cDNA was observed. SX9 is a novel dna-pkcs-deficient cell line.},
 pages = {13058--13064},
 title = {Murine cell line SX9 bearing a mutation in the dna-pkcs gene exhibits aberrant V(D)J recombination not only in the coding joint but also in the signal joint.},
 volume = {273},
 year = {1998}
}