@article{oai:repo.qst.go.jp:00044361,
 author = {Fukumura, Ryuutarou and Takahashi, Hirokazu and Saito, Toshiyuki and Tsutsumi, Yoko and Fujimori, Akira and Sato, Shinji and Tatsumi, Kouichi and Araki, Ryoko and Abe, Masumi and 福村 龍太郎 and 高橋 宏和 and 齋藤 俊行 and 藤森 亮 and 巽 紘一 and 荒木 良子 and 安倍 真澄},
 issue = {16},
 journal = {Nucleic Acids Research},
 month = {},
 note = {We have developed an AFLP-based gene expression profiling method called 'high coverage expression profiling'(HiCEP) analysis. By making improvements to the selective PCR technique we have reduced the rate of false positive peaks to 4% and consequently the number of peaks, including overlapping peaks, has been markedly decreased. As a result we can determine the relationship between peaks and original transcripts unequivocally. This will make it practical to prepare a database of all peaks, allowing gene assignment without having to isolate individual peaks. This precise selection also enables us to easily clone peaks of interest and predict the corresponding gene for each peak in some species. The procedure is highly reproducible and sensitive enough to detect even a 1.2-fold difference in gene expression. Most importantly, the low false positive rate enables us to analyze gene expression with wide coverage by means of four instead of six nucleotide recognition site restriction enzymes for fingerprinting mRNAs. Therefore, the method detects 70-80% of all transcripts, including non-coding transcripts, unknown and known genes. Moreover, the method requires no sequence information and so is applicable even to eukaryotes for which there is no genome information available.},
 title = {A sensitive transcriptome analysis method that can detect unknown transcripts},
 volume = {31},
 year = {2003}
}