{"created":"2023-05-15T14:34:18.758727+00:00","id":44358,"links":{},"metadata":{"_buckets":{"deposit":"a1f80e33-6178-484b-b217-6404110cc07d"},"_deposit":{"created_by":1,"id":"44358","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44358"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044358","sets":["1"]},"author_link":["440682","440691","440681","440685","440679","440680","440683","440684","440678","440690","440687","440692","440686","440677","440688","440689"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"44418","bibliographicPageStart":"44413","bibliographicVolumeNumber":"276","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two major complementary double-strand break (DSB) repair pathways exist in vertebrates, homologous recombination (HR), which involves Rad54, and non-homologous end-joining, which requires the DNA-dependent protein kinase (DNA-PK). DNA-PK comprises a catalytic subunit (DNA-PKcs) and a DNA-binding Ku70 and Ku80 heterodimer. To define the activities of individual DNA-PK components in DSB repair, we targeted the DNA-PKcs gene in chicken DT40 cells. DNA-PKcs deficiency caused a DSB repair defect that was, unexpectedly, suppressed by KU70 disruption. We have shown previously that genetic ablation of Ku70 confers RAD54-dependent radioresistance on S-G(2) phase cells, when sister chromatids are available for HR repair. To test whether direct interference by Ku70 with HR might explain the Ku70(-/-)/DNA-PKcs(-/-/-) radioresistance, we monitored HR activities directly in Ku- and DNA-PKcs-deficient cells. The frequency of intrachromosomal HR induced by the I-SceI restriction enzyme was increased in the absence of Ku but not of DNA-PKcs. Significantly, abrogation of HR activity by targeting RAD54 in Ku70(-/-) or DNA-PKcs(-/-/-) cells caused extreme radiosensitivity, suggesting that the relative radioresistance seen with loss of Ku70 was because of HR-dependent repair pathways. Our findings suggest that Ku can interfere with HR-mediated DSB repair, perhaps competing with HR for DSB recognition.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukushima, Toru"}],"nameIdentifiers":[{"nameIdentifier":"440677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takata, Minoru"}],"nameIdentifiers":[{"nameIdentifier":"440678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morrison, Ciaran"}],"nameIdentifiers":[{"nameIdentifier":"440679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Araki, Ryoko"}],"nameIdentifiers":[{"nameIdentifier":"440680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"440681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Masumi"}],"nameIdentifiers":[{"nameIdentifier":"440682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsumi, Kouichi"}],"nameIdentifiers":[{"nameIdentifier":"440683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jasin, Maria"}],"nameIdentifiers":[{"nameIdentifier":"440684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Dhar, Pawankumar"}],"nameIdentifiers":[{"nameIdentifier":"440685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sonoda, Eiichiro"}],"nameIdentifiers":[{"nameIdentifier":"440686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chiba, Tsutomu"}],"nameIdentifiers":[{"nameIdentifier":"440687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeda, Shunichi"}],"nameIdentifiers":[{"nameIdentifier":"440688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒木 良子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"440689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"440690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安倍 真澄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"440691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"440692","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-28"},"publish_date":"2006-06-28","publish_status":"0","recid":"44358","relation_version_is_last":true,"title":["Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:16:24.230016+00:00"}