@article{oai:repo.qst.go.jp:00044327,
 author = {Haradahira, Terushi and Okauchi, Takashi and Maeda, Jun and Zhang, Ming-Rong and Nishikawa, Touru and Suzuki, Kazutoshi and Suhara, Tetsuya and 原田平 輝志 and 岡内 隆 and 前田 純 and 張 明栄 and 鈴木 和年 and 須原 哲也},
 journal = {Synapse},
 month = {Dec},
 note = {A positron-emitter (carbon-11) labeled antagonist for the glycine-binding site of NMDA receptors, [11C]L-703,717, has a unique in vivo binding characteristic, in which it preferentially binds to cerebellar-specific NMDA receptors consisting of GluRe3 subunit and eventually accumulates in rodent cerebellum under in vivo conditions, but not under in vitro conditions.  In order to understand the in vivo-specific site and subunit localization of this radioligand, we examined the effect of endogenous glycine site agonists, glycine and D-serine, on in vivo [11C]L-703,717 binding.  An increase in extracellular glycine concentration by treatment with a glycine transporter 1 (GlyT1)-selective inhibitor, NFPS ethyl ester, significantly decreased the cerebellar localization of [11C]L-703,717 in rats.  D-serine is known to be concentrated in mammalian forebrain regions.  The lack of D-serine detection in the cerebellum may be due to the fact that it has the highest enzymatic activity of D-amino acid oxidase (DAO).  It was found that the cerebellar localization of [11C]L-703,717 is greatly diminished in mutant mice lacking DAO, in which D-serine content in the cerebellum is drastically increased from a nondetectable level in normal mice.  These studies indicate that [11C]L-703,717 is susceptible to inhibition by glycine site agonists in its in vivo binding, and suggest that regional differences in inhibitions by endogenous agonists may be a crucial factor in the site- and subunit-specific binding of this glycine-site antagonist.},
 pages = {130--136},
 title = {Effects of Endogenous Agonists, Glycine and D-Serine, on In Vivo Specific Binding of [11C]L-703,717, a PET Radioligand for the Glycine-Binding Site of NMDA Receptors},
 volume = {50},
 year = {2003}
}