{"created":"2023-05-15T14:33:59.783240+00:00","id":44081,"links":{},"metadata":{"_buckets":{"deposit":"c8ddbcd3-79f6-4f0e-a703-e4870eebc651"},"_deposit":{"created_by":1,"id":"44081","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44081"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044081","sets":["1"]},"author_link":["438217","438214","438220","438218","438215","438219","438216"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"549","bibliographicPageStart":"537","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"European Journal of Cell Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The slaty (slt) mutation is known to reduce the activity of dopachrome tautomerase (Dct)/tyrosinase (Tyr)-related protein (TRP)-2 in melanocytes. However, it is unknown whether the reduced Dct activity leads to a defect in the proliferation and differentiation of mouse melanocytes. To address this point, the proliferation and differentiation of neonatal melanocytes from congenic mice in serum-free primary culture were investigated in detail. The proliferation of slaty epidermal melanoblasts/melanocytes in culture did not differ from that of wild-type mice. However, the differentiation was greatly inhibited. Tyr activity detected by dopa reaction as well as staining of Dct/TRP-2 in slaty melanocytes was greatly reduced. The content of eumelanin in cultured slaty melanocytes was reduced, whereas the content of pheomelanin in media derived from cultured 7.5-day-old slaty melanocytes was greatly increased. The contents of eumelanin and pheomelanin in the neonatal slaty epidermis and dermis were reduced, except that the pheomelanin content in 3.5-day-old dermis was increased. These results suggest that the slaty gene affects both eumelanin and pheomelanin synthesis in developmental stage-specific and skin site-specific manners, and, in addition, the gene controls the differentiation of melanocytes via the regulation of activities of Dct/TRP-2 and Tyr.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0171-9335","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"438214","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Kazumasa"}],"nameIdentifiers":[{"nameIdentifier":"438215","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Shosuke"}],"nameIdentifiers":[{"nameIdentifier":"438216","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawa, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"438217","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizoguchi, Masako"}],"nameIdentifiers":[{"nameIdentifier":"438218","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"438219","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438220","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The slaty mutation affects eumelanin and pheomelanin synthesis in mouse melanocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The slaty mutation affects eumelanin and pheomelanin synthesis in mouse melanocytes"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-05-20"},"publish_date":"2006-05-20","publish_status":"0","recid":"44081","relation_version_is_last":true,"title":["The slaty mutation affects eumelanin and pheomelanin synthesis in mouse melanocytes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:19:39.471503+00:00"}