@article{oai:repo.qst.go.jp:00043918,
 author = {Suzuki, Gen and Nakagawa, Kenichi and Saito, Toshiyuki and Mita, Kazuei and Shirasawa, Takuji and et.al and 鈴木 元 and 中川 憲一 and 齋藤 俊行 and 三田 和英},
 journal = {International Immunology},
 month = {},
 note = {SDF-1 is a member of the CXC chemokines. In contrast to other chemokines that are induced by inflammation, SDF-1 is constitutively produced by stromal cells. In order to investigate the physiological roles of SDF-1, we constructed a fusion protein, SDF-1-Cgamma1, composed from murine SDF-1alpha and the constant region of human IgG. SDF-1-Cgamma1 stained EL-4 T lymphoma cells and the staining was blocked by rhSDF-1beta. The expression levels of SDF-1R altered along with the T cell maturation. Most c-kit+ hematopoietic precursors in fetal liver in gestational day (GD) 14.5 embryo were SDF-1R-, while c-kit+ double-negative (DN) thymocytes in the embryo were positive for SDF-1R. The receptor expression increased along with T cell maturation up to double-positive (DP) cell stage. Interestingly, SDF-1R expression was down-modulated after positive selection; CD69+CD3hi DP and CD3hi single-positive thymocytes were SDF-1R-/lo. Northern blot analysis demonstrated that SDF-1 and CXCR4 mRNAs were abundantly expressed in the thymuses of embryo and adult mice. These results demonstrate that SDF-1R expression is involved in T cell development in the thymus, particularly in positive selection.},
 pages = {1049--1056},
 title = {Loss of SDF-1 receptor expression during positive selection in the thymus.},
 volume = {10},
 year = {1998}
}