@article{oai:repo.qst.go.jp:00043770,
 author = {K., Sundaray J. and Ota, Kohei and Yamaguchi, Akihiko and Suzuki, Keiko and Matsuyama, Michiya and 鈴木 桂子},
 journal = {Fish Physiology and Biochemistry},
 month = {},
 note = {The wrasse, Pseudolabrus sieboldi, is a diandric protogynous labrid fish.  Spawning is performed by a terminal phase (TP) male and an initial phase (IP) female between 6:00 and 9:00 h daily during two-month-long spawning season. In the present study, to investigate the roles of steroid hormones in the diurnal spermatogenesis of the P. sieboldi TP male, all steroid hormones produced in the testis were identified and the synthetic pathways of these steroids were determined. Furthermore, the circulating levels of the major steroids produced were analyzed throughout a day at 3-hour intervals during spawning season. In the testis, 11-ketotestosterone (11-KT), estradiol-17beta (E2), 17,20beta-dihydroxy-4-pregnene-3-one (17,20beta-P) and 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-S) were synthesized as the major metabolites. In vitro steroid biosynthesis experiments showed similar results to the circulation profiles of the major steroids. This study is the first to clarify the complete steroidogenic pathways in the gonads of a diandric protogynous species throughout its life, when combined with the results of the steroidogenesis in the ovarian follicles. This is also the first report of a clear diurnal rhythm of the steroid production corresponding to the spermatogenic process in the testis of a male teleost.},
 pages = {193--195},
 title = {Diurnal rhythm of steroid biosynthesis in the testis of terminal phase male of protogynous wrasse, Pseudolabrus sieboldi, a daily spawner.},
 volume = {28},
 year = {2003}
}