{"created":"2023-05-15T14:33:44.310482+00:00","id":43766,"links":{},"metadata":{"_buckets":{"deposit":"caf4c510-afa4-4d85-bc48-8b301d143d3b"},"_deposit":{"created_by":1,"id":"43766","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43766"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043766","sets":["1"]},"author_link":["435171","435173","435170","435172","435168","435169"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"318","bibliographicPageStart":"311","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The growth arrest and DNA damage-inducible protein 45alpha(GADD45a)gene is responsive to a variety of DNA-damaging agents. It is known that induction of the GADD45a gene is regulated in a p53-dependent manner after ionizing irradiation. Our previous study showed that X-ray irradiation increased the transcription rate of the GADD45a gene much earlier than the maximum accumulation of stabilized p53 protein in human myeloblastic leukemia ML-1 cells. We hypothesized that some transcription factor(s) may cooperate with p53 in regulating the GADD45a gene early after the irradiation of ML-1 cells. This idea is supported by recent studies showing that the p53-dependent activation of several genes in human and mouse cells requires some additional transcription factors, such as Sp1, GKLF, Ets1, and IRF-1. To examine the possible involvement of cooperating factors in transcriptional regulation of the GADD45a gene by ionizing radiation, we complehensively searched for the X-ray-inducible binding locus of the nuclear factor throughout the upstream region (-2244 bp/+89 bp) and the third intron (+1389 bp/+2488 bp) of the GADD45a gene by EMSA using 136 probes. The X-ray-responsive binding of nuclear factors was detected at eight loci. Oct, NF-kappaB, HNF, NF-AT, and KLF family transcription factors were identified by a competition assay. It is possible that some of these factors cooperate with p53 to mediate transcriptional regulation of the GADD45a gene after ionizing irradiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Daino, Kazuhiro"}],"nameIdentifiers":[{"nameIdentifier":"435168","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichimura, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"435169","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"435170","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"臺野 和広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"435171","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"435172","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"435173","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-08-11"},"publish_date":"2005-08-11","publish_status":"0","recid":"43766","relation_version_is_last":true,"title":["Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:23:18.392306+00:00"}