@article{oai:repo.qst.go.jp:00043765,
 author = {Takahashi, Akihisa and Ota, I and Tamamoto, Tetsurou and Asakawa, Isao and Nagata, Y and Nakagawa, H and Kondo, N and Ohnishi, Ken and Furusawa, Yoshiya and Matsumoto, Hideki and Oonishi, Takeo and 高橋 昭久 and 玉本 哲郎 and 浅川 勇雄 and 大西 健 and 古澤 佳也 and 松本 英樹 and 大西 武雄},
 issue = {2},
 journal = {International Journal of Hyperthermia},
 month = {Apr},
 note = {To elucidate p53-dependency on combined treatment with radiation and hyperthermia, growth inhibition and apoptosis were analysed using transplantable human tumour. Human head and neck squamous cell carcinoma (HNSCC) cells carrying different p53 genes were transplanted into the thigh of nude mice. When the mean diameter of tumour reached 5-6 mm, the tumours were exposed to X-rays (2 Gy) or Carbon-ion (C-) beams (1 Gy) followed by heating at 42 degrees C for 20 min. Tumour growth inhibition was evaluated by measuring the diameters of tumour. The induction of apoptosis and accumulation of apoptosis-related proteins were also analysed by immunohistochemical staining. Synergistic enhancement of tumour growth inhibition by hyperthermia was observed in wild-type p53 tumours treated with X-rays or C-beams but not in mutant p53 tumours. The incidence of apoptotic cells and activated-caspase-3-positive cells after combined treatment with them were significantly high in wild-type p53 tumours compared with that in mutant p53 tumours. The hyperthermic enhancement of tumour growth inhibition by X-ray- or C-beam-irradiation was p53-dependent, suggesting that it might be highly correlated with p53-dependent apoptosis.},
 pages = {145--153},
 title = {p53-dependent hyperthermic enhancement of tumour growth inhibition by X-ray or carbon-ion beam irradiation},
 volume = {19},
 year = {2003}
}