{"created":"2023-05-15T14:33:42.033364+00:00","id":43716,"links":{},"metadata":{"_buckets":{"deposit":"298e21f4-2876-4701-bd18-0016cd7d3433"},"_deposit":{"created_by":1,"id":"43716","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43716"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043716","sets":["1"]},"author_link":["434665","434662","434661","434660","434663","434664"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"84","bibliographicPageStart":"173","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The skin is an external organ that is most frequently exposed to radiation. High-dose radiation initiates and promotes skin cancer and acute radiation injury. It is important to investigate the influence of high-dose radiation exposure on the skin at the molecular level to understand acute radiation injury. To identify genes that are associated with injury caused by high-dose radiation exposure of the skin, we used microarray technology to examine the effect of irradiation on approximately 1000 genes in normal human epidermal keratinocytes at 3 h postirradiation with a cytotoxic dose of X-ray (5 Gy). We found that 16 and 59 genes were up- and down-regulated respectively in the keratinocytes. Several apoptosis-related genes, for example, BAK and TSC-22, and anti-proliferative genes, for example, BTG-1 and BTG-3, were up-regulated. We focused on ATF3 because ATF3 is induced most strongly by X-irradiation, and its function in keratinocytes is unknown. The induction of the ATF3 mRNA and protein in keratinocytes following X-ray was confirmed by RT-PCR and western blot analysis. ATF3 was also induced and accumulated within the nuclei of keratinocytes after X-ray irradiation in vivo and in vitro. Exogenous EYFP-ATF3 also accumulated within the nuclei of keratinocytes. In the transient expression assay, EYFP-ATF3, but not EYFP, induced apoptosis in keratinocytes. Taken together, these results suggest that ATF3 plays a role in apoptosis in keratinocytes and is associated with skin injury caused by ionizing radiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koike, Manabu"}],"nameIdentifiers":[{"nameIdentifier":"434660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shiomi, Tomoe"}],"nameIdentifiers":[{"nameIdentifier":"434661","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Aki"}],"nameIdentifiers":[{"nameIdentifier":"434662","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 学","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434663","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"潮見 友江","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434664","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 亜紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"434665","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of Skin Injury-related Genes Induced by Ionizing Radiation in Human Keratinocytes using cDNA Microarray.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of Skin Injury-related Genes Induced by Ionizing Radiation in Human Keratinocytes using cDNA Microarray."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-07-06"},"publish_date":"2005-07-06","publish_status":"0","recid":"43716","relation_version_is_last":true,"title":["Identification of Skin Injury-related Genes Induced by Ionizing Radiation in Human Keratinocytes using cDNA Microarray."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:23:56.160488+00:00"}