@article{oai:repo.qst.go.jp:00043667,
 author = {Kito, Seiji and Hayao, Tatsuo and Noguchi, Yoshiko and Ohta, Yuki and Uhara, Hideki and Tateno, Shintarou and 鬼頭 靖司 and 早尾 辰雄 and 太田 有紀 and 舘野 真太郎},
 issue = {5},
 journal = {Comparative Medicine},
 month = {Oct},
 note = {We examined in vitro fertilizability and development of 10 inbred mouse strains C57BL/6J, C57BL/10, C57BL/10.D2/newSn, C57BL/10-Thy1.1, C57BL/10.Br/Sn, C3H/He, RFM/Ms, STS/A, BALB/c-nu and C.B-17/Icr), and the viability of frozen-thawed in vitro fertilized (IVF) embryos after embryo transfer (ET). In seven strains, fertilizability was significantly greater in modified human tubal fluid (mHTF) compared with modified Krebs-Ringer's bicarbonate solution (TYH medium). The TYH medium supported almost no fertilization in four strains. More than 80% of IVF embryos developed to the blastocyst stage by 120 h in potassium-enhanced simplex optimization medium (KSOM). Reciprocal fertilization between C57BL/6J and BALB/c-nu gametes in TYH medium yielded poor fertilization of BALB/c-nu due to spermatozoal deficiencies. Increased concentrations of bovine serum albumin and spermatozoa during capacitation and Percoll washing did not drastically affect fertilization. The mHTF, but not TYH medium, supported BALB/c-nu spermatozoa penetration into the zona pellucida irrespective of capacitation media. In vitro fertilized embryos frozen-thawed rapidly were transferred to surrogate mothers at the two-cell stage. Compared with that of unfrozen controls, rapid freezing had no significant effect on fetus development except in C57BL/10.D2/newSn mice. These results suggest that mHTF medium is superior with respect to IVF of inbred mice, and that KSOM adequately supports in vitro fertilized embryo development in inbred mice. The data also indicate that rapid freezing of pronucleate embryos following IVF is suitable for cryopreservation and embryo banking of inbred mice and for the production of genetically modified mice.},
 pages = {564--570},
 title = {Improved in Vitro Fertilization and Development by Use of Modified Human Tubal Fluid and Applicability of Pronucleate Embryos for Cryopreservation by Rapid Freezing in Inbred Mice},
 volume = {54},
 year = {2004}
}