@article{oai:repo.qst.go.jp:00043467, author = {Kito, Seiji and Noguchi, Yoshiko and Oota, Yuki and 鬼頭 靖司 and 太田 有紀}, journal = {Experimental Animals}, month = {Jan}, note = {Response of in vitro fertilized embryos against oxygen (5% or air) and the half strengths of the MEM amino acids were compared between in-house bred C57BL/6JNrs and commercially available C57BL/6JSlc mice. Under atmospheric oxygen, development to blastocysts and expanded blastocyst and nuclear number are lower in Slc embryos than in Nrs embryos and Slc embryos did not increase nuclear number during 72-96 hr culture. Amino acid did not necessarily have beneficial effect on embryo development and nuclear number of Slc embryos, but on nuclear number of Nrs embryos. Because present study showed differences in sensitivity to culture conditions between the C57BL/6J substrains, care must be taken in embryo manipulation of this inbred strain to study embryo development or other studies.}, pages = {63--66}, title = {Developmental responses of two substrains of in vitro fertilized C57BL/6J mouse embryos to oxygen and amino acids}, volume = {52}, year = {2003} }