@article{oai:repo.qst.go.jp:00043420,
 author = {Hirobe, Tomohisa and Furuya, Rikako and Akiu, Satoru and Ifuku, Ouji and Fukuda, Minoru and 廣部 知久},
 issue = {5},
 journal = {Pigment Cell Research},
 month = {Oct},
 note = {Long-term exposure of ultraviolet radiation B (UVB) induced pigmented spots in the dorsal skin of hairless mice of Hos:(HR-1 X HR/De) F1. Previous study showed that the proliferative and differentiative activities of cultured epidermal melanoblasts/melanocytes from UVB-induced pigmented spots increased with the development of the pigmented spots. To determine whether the increase in the proliferative and differentiative activities of epidermal melanoblasts/melanocytes was brought about by direct changes in melanocytes, or by indirect changes in surrounding keratinocytes, pure cultured melanoblasts/melanocytes and keratinocytes were prepared and co-cultured in combination between control and irradiated mice in a serum-free culture medium. Keratinocytes from irradiated mice stimulated the proliferation and differentiation of both neonatal and adult non-irradiated melanoblasts/melanocytes more greatly than those from adult non-irradiated mice. In contrast, both non-irradiated and irradiated adult melanocytes proliferated and differentiated similarly when they were co-cultured with irradiated adult keratinocytes. These results suggest that the increased proliferative and differentiative activities of mouse epidermal melanocytes from UVB-induced pigmented spots are regulated by keratinocytes, rather than melanocytes.},
 pages = {391--399},
 title = {Keratinocytes control the proliferation and differentiation of cultured epidermal melanocytes from ultraviolet radiation B-induced pigmented spots in the dorsal skin of hairless mice},
 volume = {15},
 year = {2002}
}