{"created":"2023-05-15T14:33:28.601315+00:00","id":43418,"links":{},"metadata":{"_buckets":{"deposit":"83a642b3-c5e8-41d6-8df1-4c8bbe2a23f9"},"_deposit":{"created_by":1,"id":"43418","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43418"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043418","sets":["1"]},"author_link":["431809","431810","431812","431811","431807","431808"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"366","bibliographicPageStart":"351","bibliographicVolumeNumber":"292","bibliographic_titles":[{"bibliographic_title":"Journal of Experimental Zoology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cells positive to the dopa reaction (melanocytes) as well as to the combined dopa-premelanin reaction (melanoblasts and melanocytes) in the epidermis of C57BL/10JHir-p/p  (pink-eyed dilution) mice were fewer and less reactive than in C57BL/10JHir (black, P/P) mice, suggesting that the proliferation and differentiation of p/p melanocytes are inhibited. To confirm the inhibitory effects of p gene on the proliferation and differentiation of epidermal melanocytes, we cultured epidermal cell suspensions of neonatal skins from P/P and p/p in a serum-free medium. The proliferation and differentiation of p/p melanoblasts/melanocytes in primary culture were greatly inhibited as compared to P/P melanoblasts/melanocytes. The morphology of p/p melanoblasts/melanocytes cultured in melanocyte growth medium, though non-pigmented, was similar to P/P melanocytes; namely, dendritic, polygonal, or epithelioid. About 8% of p/p cells cultured in melanocyte growth medium were positive to the dopa reaction, and about 25% were reactive to the combined dopa-premelanin reaction. Eumelanin content in p/p was extremely reduced compared to P/P. The immunocytochemical staining of p/p melanoblasts/melanocytes revealed that they are negative to tyrosinase, but reactive to tyrosinase-related protein (TRP)-1, TRP-2, and c-kit. However, the reactivities in p/p were lower than in P/P . Although the differentiation of p/p melanoblasts was not induced by endothelin (ET)-1, ET-2, and ET-3, the proliferation of p/p melanoblasts was stimulated by them. These results suggest for the first time that p gene exerts its influence on the proliferative activities of mouse epidermal melanoblasts by affecting the regulatory mechanisms dependent on the function of ETs.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-104X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"431807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawa, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"431808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizoguchi, Masako"}],"nameIdentifiers":[{"nameIdentifier":"431809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Shosuke"}],"nameIdentifiers":[{"nameIdentifier":"431810","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Kazumasa"}],"nameIdentifiers":[{"nameIdentifier":"431811","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431812","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of genic substitution at the pink-eyed dilution locus on the proliferation and differentiation of mouse epidermal melanocytes in vivo and in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of genic substitution at the pink-eyed dilution locus on the proliferation and differentiation of mouse epidermal melanocytes in vivo and in vitro"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-06-18"},"publish_date":"2004-06-18","publish_status":"0","recid":"43418","relation_version_is_last":true,"title":["Effects of genic substitution at the pink-eyed dilution locus on the proliferation and differentiation of mouse epidermal melanocytes in vivo and in vitro"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:27:32.097988+00:00"}