{"created":"2023-05-15T14:33:28.513272+00:00","id":43416,"links":{},"metadata":{"_buckets":{"deposit":"ecc5a631-970f-4420-8a3c-20ef47a3a8d7"},"_deposit":{"created_by":1,"id":"43416","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43416"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043416","sets":["1"]},"author_link":["431800","431801","431802","431803","431798","431804","431799"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"172","bibliographicPageStart":"162","bibliographicVolumeNumber":"191","bibliographic_titles":[{"bibliographic_title":"Journal of Cellular Physiology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The epidermal cell suspensions of the neonatal dorsal skin derived from wild type mouse at the pink-eyed dilution (p) locus (black, C57BL/10JHir-P/P) and their congenic mutant mouse (pink-eyed dilution, C57BL/10JHir-p/p) were cultured with a serum-free melanocyte growth medium supplemented with additional L-tyrosine (Tyr) from initiation of the primary culture. L-Tyr inhibited the proliferation of P/P melanocytes in a dose-dependent manner, whereas L-Tyr stimulated the proliferation of p/p melanoblasts and melanocytes regardless of dose. On the other hand, L-Tyr stimulated (P/P) or induced (p/p) the differentiation of epidermal melanocytes in a dose-dependent manner.  In both P/P and p/p melanoblasts and melanocytes cultured with 2.0 mM L-Tyr for 14 days, slight increases in contents of eumelanin marker, pyrrole-2,3,5-tricarboxylic acid (PTCA) and pheomelanin marker, aminohydroxyphenylalanine (AHP) were observed. The average number of total melanosomes (stages I, II, III and IV)  per P/P melanocyte was not changed by L-Tyr treatment, but the proportion of stage IV melanosomes in the total melanosomes was increased. On the contrary, in p/p melanoblasts and melanocytes L-Tyr increased dramatically the number of stage II, III and IV melanosomes as well as the proportion of stage III melanosomes. Contents of PTCA and eumelanin precursor, 5,6-dihydroxyindole-2-carboxylic acid (DHICA) of cultured media in p/p melanocytes were much more greatly increased than in P/P melanocytes. However, contents of AHP and pheomelanin precursor, 5-S-cysteinyldopa (5-S-CD) of cultured media in p/p melanocytes were increased in a similar tendency to P/P melanocytes. These results suggest that p/p melanocytes in the primary culture are induced to synthesize eumelanin by excess L-Tyr, but difficult to accumulate them in melanosomes.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9541","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"431798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Kazumasa"}],"nameIdentifiers":[{"nameIdentifier":"431799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Shosuke"}],"nameIdentifiers":[{"nameIdentifier":"431800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"431801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawa, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"431802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizoguchi, Masako"}],"nameIdentifiers":[{"nameIdentifier":"431803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431804","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stimulation of the proliferation and differentiation of mouse pink-eyed dilution epidermal melanocytes by excess tyrosine in serum-free primary culture","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stimulation of the proliferation and differentiation of mouse pink-eyed dilution epidermal melanocytes by excess tyrosine in serum-free primary culture"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-06-18"},"publish_date":"2004-06-18","publish_status":"0","recid":"43416","relation_version_is_last":true,"title":["Stimulation of the proliferation and differentiation of mouse pink-eyed dilution epidermal melanocytes by excess tyrosine in serum-free primary culture"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:27:33.971461+00:00"}