{"created":"2023-05-15T14:33:28.422989+00:00","id":43414,"links":{},"metadata":{"_buckets":{"deposit":"b668eb35-0bfd-4c19-89e8-571baeeb7a5b"},"_deposit":{"created_by":1,"id":"43414","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43414"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043414","sets":["1"]},"author_link":["431790","431793","431791","431792"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"628","bibliographicPageStart":"619","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"Pigment Cell Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Changes in the proliferation and differentiation of epidermal melanocytes derived from newborn mice wild-type at the pink-eyed dilution (p) locus (P/P) and from congenic mice mutant at that locus (p/p) were investigated in serum-free primary culture, with or without the addition of L-Tyr. Incubation with added L-Tyr inhibited the proliferation of P/P melanocytes in a concentration-dependent manner and that inhibition was gradually augmented as the donor mice aged. In contrast, L-Tyr stimulated the proliferation of p/p melanoblasts/melanocytes derived from 0.5 -day-old mice, but inhibited their proliferation when derived from 3.5- or from 7.5-day-old mice. L-Tyr stimulated the differentiation of P/P melanocytes.  However, almost all cells were undifferentiated melanoblasts in control cultures derived from 0.5-, 3.5- and 7.5-day-old p/p mice, but L-Tyr induced their differentiation as the age of the donor mice advanced. The content of the eumelanin marker, pyrrole-2,3,5-tricarboxylic acid (PTCA) as well as the pheomelanin marker, 4-amino-3-hydroxyphenylalanine (4-AHP) in p/p melanocytes was greatly reduced compared with P/P melanocytes. However, the content of eumelanin and its precursor, 5,6-dihydroxyindole-2-carboxylic acid (DHICA), as well as the content of pheomelanin and its precursor, 5-S-cysteinyldopa (5-S-CD) in culture media from p/p melanocytes were similar to those of P/P melanocytes at all ages tested. L-Tyr increased the content of eumelanin and pheomelanin 2- to 3-fold in cultured cells and in media derived from 0.5-, 3.5- and 7.5-day-old mice. These results suggest that the proliferation of p/p melanoblasts/melanocytes is stimulated by L-Tyr, and that the differentiation of melanocytes is induced by L-Tyr as the age of the donor mice advanced, even though eumelanin and pheomelanin fail to accumulate in p/p melanocytes and are released from them at all ages of skin development.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0893-5785","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"431790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Kazumasa"}],"nameIdentifiers":[{"nameIdentifier":"431791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Shosuke"}],"nameIdentifiers":[{"nameIdentifier":"431792","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431793","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Changes in the Proliferation and Differentiation of Neonatal Mouse Pink-Eyed Dilution Melanocytes in the Presence of Excess Tyrosine","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Changes in the Proliferation and Differentiation of Neonatal Mouse Pink-Eyed Dilution Melanocytes in the Presence of Excess Tyrosine"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-06-18"},"publish_date":"2004-06-18","publish_status":"0","recid":"43414","relation_version_is_last":true,"title":["Changes in the Proliferation and Differentiation of Neonatal Mouse Pink-Eyed Dilution Melanocytes in the Presence of Excess Tyrosine"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:27:36.063804+00:00"}