@article{oai:repo.qst.go.jp:00043373, author = {Kazuaki, Matsui and Ishii, Nobuyoshi and Kawabata, Zenichiro and 石井 伸昌}, issue = {4}, journal = {Applied and Environmental Microbiology}, month = {May}, note = {We studied the effects of cocultivation with either Euglena Gracilis (Euglenophyta), Microcystis aeruginosa (Cyanophyta), Chlamydonomas neglecta (Chlorophyta), or Carteria inversa (Chlorophyta) on the production of extracellular plasmid DNA by Excherichia coli LE392(pKZ105), Dot blot hybridization analysis showed a significant release of plasmid DNA by cocultivation with all the algae tested. Further analysis by electrotransformation confirmed the release of transformable plasmid DNA by cocultivation with either E.Gracilis, M. Aeruginosa, or C. inversa. These results suggest algal involvement in bacterial horizontal gene tranfer by stimulating the release of transformable DNA into aquatic environments.}, pages = {2399--2404}, title = {Release of Extracellular Transformable Plasmid DNA from Escherichia coli Cocultivated with Algae}, volume = {69}, year = {2003} }