{"created":"2023-05-15T14:33:26.244204+00:00","id":43365,"links":{},"metadata":{"_buckets":{"deposit":"5c7f8ec0-fd3a-4d4b-8173-929618d7302d"},"_deposit":{"created_by":1,"id":"43365","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43365"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043365","sets":["1"]},"author_link":["431398","431392","431393","431395","431397","431390","431394","431389","431391","431396"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"154","bibliographicPageStart":"149","bibliographic_titles":[{"bibliographic_title":"Genes and Genetic Systems"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Previously,the allelic expansion of a 33-bp AT-rich minisatellite repeat has been reported to cause FRA16B,a distamycin A-inducible fragile site.  Here,we identified a novel 35-bp minisatellite repeat at FRA16B in a Japanese carrier.  The nucleotide sequence of the 35-bp minisatellite was highly AT-rich and nearly identical to the 33-bp one but with insertion of two uncleotides,thymine and adenine.  The copy number of the AT-rich minisatellite was 21 in total in the carrier,while only a few copies of the 33-bp minisatellite were present in a non-carrier Japanese subject.  These results suggest that the molecular mechanism involved in the allelic expansion of the minisatelite repeat in FRA16B recognizes both minisatellites,the 33-bp one and the 35-bp one,as an amplicon.  These observations were different from the ones at folate-sensitive fragile sites,where the CCG triplet repeat was commonly involved in the allelic expansion.  Although a slight reduction in AT content (95%>90%) in the region of minisatellite expansion in the carrier subject was observed,both AT-content and length of the highly AT-ruch region seem to play important roles in the cytogenetic expression of the distamycin A-inducible fragile site.  In another normal subject,without fragile site expression,allelic expansion involving the 33-bp minisatellite was observed,and the length of the AT-rich DNA region was increased up to 1,000 bp.  Since the length of the AT-rich minisatellite region was increased up to 1,100-bp in the carrier subject,the threshold length for the cytogenetic expression of the AT-rich DNA region may be between about 1,000-bp and 1,100-bp.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1341-7568","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Masatake"}],"nameIdentifiers":[{"nameIdentifier":"431389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Satsuki"}],"nameIdentifiers":[{"nameIdentifier":"431390","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mita, Kazuei"}],"nameIdentifiers":[{"nameIdentifier":"431391","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"431392","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morimyo, Mitsuoki"}],"nameIdentifiers":[{"nameIdentifier":"431393","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内 正剛","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431394","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 さつき","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431395","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田 和英","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431396","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 俊行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431397","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森明 充興","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431398","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel minisatellite repeat expansion identified at FRA16B in a Japanese carrier","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel minisatellite repeat expansion identified at FRA16B in a Japanese carrier"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-03-18"},"publish_date":"2004-03-18","publish_status":"0","recid":"43365","relation_version_is_last":true,"title":["A novel minisatellite repeat expansion identified at FRA16B in a Japanese carrier"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:28:10.878085+00:00"}