@article{oai:repo.qst.go.jp:00043361,
 author = {Suzuki, Masao and Tsuruoka, Chizuru and Kanai, Tatsuaki and Kato, Takeshi and Yatagai, Fumio and Watanabe, Masami and 鈴木 雅雄 and 鶴岡 千鶴 and 金井 達明 and 谷田貝 文夫},
 issue = {4},
 journal = {Biological Sciences in Space},
 month = {Mar},
 note = {We investigated the difference in cell-killing effect and mutation induction between carbon- and neon-ion beams in normal human cells. Carbon- and neon-ion beams were accelerated by the Riken Ring Cyclotron (RRC) at the Institute of Physical and Chemical Research in Japan. Cell-killing effect was measured as the reproductive cell death using the colony formation assay. Mutation induction at the HPRT locus was detected to measure 6-thioguanine-resistant clones. The mutation spectrum of the deletion pattern of exons of induced mutants was analyzed using the multiplex polymerase chain reaction (PCR). Cell-killing effect was almost the same between carbon- and neon-ion beams with similar linear energy transfer (LET) values, while there observed a large difference in mutation frequency. Furthermore, in the case of neon-ion beams 60% of mutants showed total deletions and 35-40% showed partial deletions, while 95-100% of carbon-ion induced mutants showed total deletions. The results suggest that different ion species may cause qualitative and quantitative difference in mutation induction even if the LET values are similar.},
 pages = {302--306},
 title = {Qualitative and quantitative difference in mutation induction between carbon- and neon-ion beams in normal human cells},
 volume = {17},
 year = {2004}
}