{"created":"2023-05-15T14:33:25.216227+00:00","id":43342,"links":{},"metadata":{"_buckets":{"deposit":"b55d3d13-4917-4d4c-b60e-b80cf064f989"},"_deposit":{"created_by":1,"id":"43342","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43342"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043342","sets":["1"]},"author_link":["431113","431114","431115"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"94","bibliographicPageStart":"883","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Microbiology and Immunology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V delta-D delta signal joints, depending on the V delta and D delta gene usage in the developmental stage. Nucleotide insertions at the V delta-D delta 1 signal joints were less frequent for the V delta 4, 5 genes preferentially utilized in adult thymocytes than for the V delta 3, 6 genes, infrequently rearranged to D delta 1. In addition to standard signal joints, unexpectedly, novel nonstandard products, \"replacement joints\" of D delta 1 substituted downstream by the recombination signal sequence of V delta were also found. However, no D delta 2-associated replacement joints other than V delta 5 were found. The other replacement joints of D delta 1-D delta 2 recombination were also observed. The mutation in TCR beta gene affected the frequency of nucleotide insertions at the V delta-D delta signal joints and inhibited the formation of replacement joint. Recombination mechanism generating the replacement joint and the possible role of TCR beta in up-regulation of TCR delta gene rearrangements are discussed.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-5600","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Muto, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"431113","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"431114","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武藤 正弘","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"431115","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"TCR delta gene rearrangements revealed by fine structure of the recombination junction in mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TCR delta gene rearrangements revealed by fine structure of the recombination junction in mice."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-02-04"},"publish_date":"2004-02-04","publish_status":"0","recid":"43342","relation_version_is_last":true,"title":["TCR delta gene rearrangements revealed by fine structure of the recombination junction in mice."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:28:26.813060+00:00"}