{"created":"2023-05-15T14:33:22.796866+00:00","id":43288,"links":{},"metadata":{"_buckets":{"deposit":"3e703921-6118-422c-b1d8-b89fb175ae60"},"_deposit":{"created_by":1,"id":"43288","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43288"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043288","sets":["1"]},"author_link":["430602","430604","430599","430605","430601","430603","430600","430598"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"55","bibliographicPageStart":"47","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"Comparative Immunology, Microbiology & Infectious Diseases"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The elucidation of the antigenic structure of the envelope proteins of Arteriviridae  which includes lactate dehydrogenase-elevating virus (LDV) will provide further understanding of a mechanism of strict host cell specificity. To analyze the linkage between LDV envelope proteins, M/VP-2 and VP-3, which may play an important role in viral infectivity, we generated specific antibody against M/VP-2 that has not been reported in previous studies. A synthetic polypeptide corresponding to the C-terminal region of LDV strain C (LDV-C) ORF6, which encodes M/VP-2, was chemically synthesized and coupled to keyhole limpet hemocyanin (KLH). The peptide was immunogenic in rabbits and induced antibody specific for viral protein. Western blotting and immunofluorescence analysis of virion M/VP-2 in infected macrophages showed that the antibody was able to react specifically with authentic virion protein. The immunoreactive antibody against LDV M/VP-2 described in this study will be useful for further studies of the specific roles of the envelope proteins in arterivirus assembly and infectivity.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0147-9571","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Omoe, Hiromi"}],"nameIdentifiers":[{"nameIdentifier":"430598","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Omoe, Katsuhiko"}],"nameIdentifiers":[{"nameIdentifier":"430599","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakaguchi, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"430600","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kameoka, Yousuke"}],"nameIdentifiers":[{"nameIdentifier":"430601","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsushita, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"430602","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inada, Toshiki"}],"nameIdentifiers":[{"nameIdentifier":"430603","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"重茂 浩美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430604","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松下 悟","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430605","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-12-02"},"publish_date":"2003-12-02","publish_status":"0","recid":"43288","relation_version_is_last":true,"title":["Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:29:05.526625+00:00"}