{"created":"2023-05-15T14:33:21.740981+00:00","id":43264,"links":{},"metadata":{"_buckets":{"deposit":"2442881e-0739-4c2c-a960-ed51ff26e005"},"_deposit":{"created_by":1,"id":"43264","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43264"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043264","sets":["1"]},"author_link":["430369","430367","430368","430372","430371","430374","430370","430373","430375"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5894","bibliographicPageStart":"5885","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"Cancer Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Reactive oxygen species play a critical role in the onset of apoptosis induced by various extracellular stimuli, including ionizing radiation. Therefore active regulation of reactive oxygen species-metabolizing enzymes may be one response to an apoptotic stimulus. In this regard, HP100 cells, H(2)O(2)-resistant variants derived from human leukemia HL60 cells, display an interesting phenotype in which the activity of catalase is constitutively high, whereas its mRNA is reduced after X-ray irradiation. In the present study, we investigated the molecular mechanisms underlying this phenomenon. By combining analyses from nuclear run-on, reporter gene transient transfection, genomic footprinting, site-directed mutagenesis, electrophoretic mobility shift analysis, and Western blotting experiments, we found that constitutively elevated catalase expression is strongly regulated at the transcriptional level by both Sp1 and CCAAT-recognizing factors and that much higher levels of nuclear Sp1 and NF-Y are present in HP100 nuclei as compared with HL60 nuclei. In addition, we demonstrated an X-ray-inducible association of a WT1/Egr-related factor with an overlapping Sp1/Egr-1 recognition sequence located within the core promoter of the catalase gene. This association may lead to inactivation of the promoter by disturbing or competing with the transactivating ability of Sp1.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0008-5472","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"430367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichimura, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"430368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mita, Kazuei"}],"nameIdentifiers":[{"nameIdentifier":"430369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yukawa, Osami"}],"nameIdentifiers":[{"nameIdentifier":"430370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"L., Cartwright Iain"}],"nameIdentifiers":[{"nameIdentifier":"430371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430372","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430373","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田 和英","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430374","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"湯川 修身","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"430375","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Regulation of the catalase gene promoter by Sp1, CCAAT-recognizing factors,","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of the catalase gene promoter by Sp1, CCAAT-recognizing factors,"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-11-14"},"publish_date":"2003-11-14","publish_status":"0","recid":"43264","relation_version_is_last":true,"title":["Regulation of the catalase gene promoter by Sp1, CCAAT-recognizing factors,"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:29:22.218286+00:00"}