@article{oai:repo.qst.go.jp:00043110,
 author = {Shao, Chunlin and Furusawa, Yoshiya and Aoki, Mizuho and Matsumoto, Hideki and Ando, Koichi and Ｓｈａｏ Ｃｈｕｎｌｉｎ and 古澤 佳也 and 青木 瑞穂 and 松本 英樹 and 安藤 興一},
 journal = {International Journal of Radiation Biology},
 month = {Sep},
 note = {Purpose: To investigate the signal factor and its function in the medium-mediated bystander effect during heavy-ion irradiation of human salivary gland (HSG) neoplastic cells.   Materials and methods: Unirradiated recipient HSG cells were co-cultivated with HSG donor cells irradiated with 290 MeV/u carbon beams having different LET values. Cell proliferation and micronucleus (MN) induction in recipient cells with and without treatment of a NO scavenger (PTIO) were measured and the concentration of nitrite in the co-culture medium was detected. As a direct control, the effects of a nitric oxide (NO) generator (sper/NO) on cell proliferation and MN induction were also examined.    Results: Increases in cell proliferation and MN induction were found in the recipient HSG cells as a result of co-culturing and cell proliferation was obviously enhanced during a further subculture. In comparison with 13 keV/um, 100 keV/um carbon-ion irradiation was found to be a more efficient inducer of the medium-mediated bystander effect. The treatment of cells by PTIO resulted in elimination of such effects, which supports a role for NO in the medium-mediated bystander effect. As an oxidization product of NO, nitrite was detected in the co-culture medium, and the dose-response for its concentration was similar to that of cell proliferation and MN induction in the recipient cells. When the HSG cells were treated by sper/NO with a concentration of less than 20 uM, cell proliferation was enhanced, whereas MN increased along with sper/NO concentration.    Conclusion: NO participated in the medium-mediated bystander effects on cell proliferation and MN induction, depending on the LET of irradiation.},
 pages = {837--844},
 title = {Nitric oxide-mediated bifunctional bystander effect induced by heavy-Ion Radiation in human salivary grand neoplastic cells},
 volume = {78},
 year = {2002}
}