@article{oai:repo.qst.go.jp:00043100,
 author = {Kinoshita, Chikako and Yaoi, Takeshi and Nojima, Kumie and Fushiki, Shinji and 矢追 毅 and 野島 久美恵 and 伏木 信次},
 journal = {Acta Histochemica et Cytochemica},
 month = {},
 note = {We report here the effects of heavy ion beam on postnatal mouse cerebellar development, with reference to cell death. Eight-day-old B6C3F1 mice were irradiated with single doses of 0.1, 0.25, 0.5, 1.0, and 2.0 Gy, using a carbon beam of 290 MeV/u delivered from a heavy ion medical accelerator in Chiba (HIMAC). To compare the effects of X-rays with those of accelerated carbon ions, 8-day-old mice were exposed to X-rays single doses of 0.1, 0.25, 0.5, 1.0, and 2.0 Gy, respectively. Pups were fixed at 1, 6, 12 and 24 hr after exposure to HIMAC beams or X-rays. Four-mm-thick parasagittal sections of the cerebella were processed for staining with the TUNEL (terminal dUTP nick-end labeling) technique. The density of fragmented unclei in the external granular layer increased with time, peaking at 6 hr after exposure, in both the HIMAC and X-irradiated groups. In the HIMAC groups, the density was significantly higher in those animals exposed to 0.25 Gy or more compared to 0 Gy, whereas in the X-irradiated groups it was significantly higher in those mice exposed to 0.5 Gy or more. Electron microscopic ezaminations revealed chromatin condensation in the first in vivo evidence that aplptotic cell death is induced in developing mouse cerebellum after exposeure to heavy ion particles and to X-rays may reflect the high linear energy transfer (LET) associated with a heavy ion beam.},
 pages = {145--151},
 title = {The Effects of Heavy Ion Particles on the Developing Murine Cerebellum, with Special Reference to Cell Death},
 volume = {36},
 year = {2003}
}