{"created":"2023-05-15T14:33:13.930767+00:00","id":43089,"links":{},"metadata":{"_buckets":{"deposit":"16be9cef-9c63-49d2-be80-598870f0d696"},"_deposit":{"created_by":1,"id":"43089","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43089"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043089","sets":["1"]},"author_link":["428733","428736","428741","428735","428737","428740","428734","428738","428739"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"914","bibliographicPageStart":"909","bibliographicVolumeNumber":"157","bibliographic_titles":[{"bibliographic_title":"Journal of Cell Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Terminal differentiation exerts a remarkably tight control on cell proliferation. However, the oncogenic products of DNA tumor viruses, such as adenovirus E1A, can force postmitotic cells to proliferate, thus representing a powerful tool to study progression into S phase. In this study, we identified the gene encoding Np95, a murine nuclear phosphoprotein, as an early target of E1A-induced transcriptional events. In terminally differentiated (TD) cells, the activation of Np95 was specifically induced by E1A, but not by overexpression of E2F-1 or of the cyclin E (cycE)-cyclin-dependent kinase 2 (cdk2) complex. In addition, the concomitant expression of Np95 and of cycE-cdk2 was alone sufficient to induce S phase in TD cells. In NIH-3T3 cells, the expression of Np95 was tightly regulated during the cell cycle, and its functional ablation resulted in abrogation of DNA synthesis. Thus, expression of Np95 is essential for S phase entry. Previous evidence suggested that E1A, in addition to its well characterized effects on the pRb/E2F-1 pathway, activates a parallel and complementary pathway that is also required for the reentry in S phase of TD cells (Tiainen, M., D. Spitkousky, P. Jansen-Durr, A. Sacchi, and M. Crescenzi. 1996. Mol. Cell. Biol. 16:5302-5312). From our results, Np95 appears to possess all the characteristics to represent the first molecular determinant identified in this pathway.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9525","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Marc, Bonapace Ian"}],"nameIdentifiers":[{"nameIdentifier":"428733","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Latella, Lucia"}],"nameIdentifiers":[{"nameIdentifier":"428734","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Papait, Roberto"}],"nameIdentifiers":[{"nameIdentifier":"428735","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nicassio, Francesco"}],"nameIdentifiers":[{"nameIdentifier":"428736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sacco, Alessandra"}],"nameIdentifiers":[{"nameIdentifier":"428737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Muto, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"428738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Crescenzi, Marco"}],"nameIdentifiers":[{"nameIdentifier":"428739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Paolo, Di Fiore Pier"}],"nameIdentifiers":[{"nameIdentifier":"428740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武藤 正弘","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428741","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-05-19"},"publish_date":"2003-05-19","publish_status":"0","recid":"43089","relation_version_is_last":true,"title":["Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:31:24.531711+00:00"}