{"created":"2023-05-15T14:33:12.663057+00:00","id":43060,"links":{},"metadata":{"_buckets":{"deposit":"ce8dd49d-00a4-4284-a596-50a81567b0f6"},"_deposit":{"created_by":1,"id":"43060","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43060"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043060","sets":["1"]},"author_link":["428492","428493","428495","428494"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"782","bibliographicPageStart":"777","bibliographicVolumeNumber":"159","bibliographic_titles":[{"bibliographic_title":"Journal of Cell Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it complemented the defect and so was functional. Photobleaching revealed two kinetic fractions of polymerase in living nuclei: less than 75% moved rapidly, but less than 25% was transiently immobile (association t1/2 20min) and transcriptionally active, as incubation with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole eliminated it. No immobile but inactive fraction was detected, providing little support for the existence of a stable holoenzyme, or the slow stepwise assembly of a preinitiation complex on promoters or the nuclear substructure. Actinomycin D decreased the rapidly moving fraction, suggesting that engaged polymerases stall at intercalated molecules while others initiate. When wild-type cells containing only the endogenous enzyme were incubated with 3H uridine nascent transcripts became saturated with tritium with similar kinetics (t1/2  14min). These data are consistent with a polymerase being mobile for one half to five sixths of a transcription cycle, and rapid assembly into the preinitiation complex. Then, most expressed transcription units would spend significant times unassociated with engaged polymerases.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9525","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kimura, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"428492","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugaya, Kimihiko"}],"nameIdentifiers":[{"nameIdentifier":"428493","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Cook, Peter"}],"nameIdentifiers":[{"nameIdentifier":"428494","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅谷 公彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428495","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The transcription cycle of RNA polymerase II in living cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The transcription cycle of RNA polymerase II in living cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-02-27"},"publish_date":"2003-02-27","publish_status":"0","recid":"43060","relation_version_is_last":true,"title":["The transcription cycle of RNA polymerase II in living cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:31:44.381761+00:00"}