{"created":"2023-05-15T14:33:07.907554+00:00","id":42952,"links":{},"metadata":{"_buckets":{"deposit":"5b206069-0685-4ba7-a03f-3fee0659e122"},"_deposit":{"created_by":1,"id":"42952","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"42952"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00042952","sets":["1"]},"author_link":["427419","427420","427429","427421","427428","427426","427425","427430","427423","427427","427422","427424"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"215","bibliographicPageStart":"201","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" A431 cells/UVC-induced apoptosis/Caspase 8/Fas/JNK/PAPK. We previously observed that p53-mutated human epithelial tumor A431 cells underwent apoptosis after ultraviolet C (UVC) irradiation through the caspases-8 and -3 pathway. Fas/FasL is known to initiate apoptosis in　several cell lines via caspase-8 activation. Then, to determine if Fas/FasL mediates apoptosis in A431. we investigated Fas expression and modulation in UVC-irradiated A431 cells. A431 constitutively expressed Fas, which gradually decreased after UVC-irradiation. Pretreatment　with a neutralizing anti-Fas antibody, ZB4, did not abrogate the UVC-induced apoptosis. An agonistic anti-Fas antibody, CH11, very slowly induced apoptosis in A431. suggesting that the constitutively expressed Fas had a low functional potential. Hence, UVC-induced apoptosis in A431 seems to occur independent of the Fas signal. Interestingly, however, a pretreatment with CH11 remarkably potentiated UVC-induced apoptosis. An inhibitor of caspase-8, Ac-IETD-CHO, partially inhibited UVC-induced apoptosis. JNK was phosphorylated immediately after exposure to UVC. prior to apoptotic chromatin condensation. Our data suggest that the activation of caspase-8 occurs independent of Fas upregulation, and that JNK/ SAPK contributes to UVC-induced apoptosis in human epithelial A431 cells.\n","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Inaba, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"427419","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hee, Choi Keun"}],"nameIdentifiers":[{"nameIdentifier":"427420","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bing, Wang"}],"nameIdentifiers":[{"nameIdentifier":"427421","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haginoya, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"427422","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"427423","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayata, Isamu"}],"nameIdentifiers":[{"nameIdentifier":"427424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohyama, Harumi"}],"nameIdentifiers":[{"nameIdentifier":"427425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"稲葉 浩子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"427426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"427427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 武","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"427428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"早田 勇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"427429","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大山 ハルミ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"427430","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fas-independent apoptosis induced by UVC in p53-mutated human epithelial tumor A431 cells through activation of caspase-8 and JNK/SAPK.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fas-independent apoptosis induced by UVC in p53-mutated human epithelial tumor A431 cells through activation of caspase-8 and JNK/SAPK."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-06-14"},"publish_date":"2002-06-14","publish_status":"0","recid":"42952","relation_version_is_last":true,"title":["Fas-independent apoptosis induced by UVC in p53-mutated human epithelial tumor A431 cells through activation of caspase-8 and JNK/SAPK."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:33:01.708273+00:00"}