@article{oai:repo.qst.go.jp:00042883,
 author = {Kito, Seiji and Tateno, Shintarou and Oota, Yuki and Noguchi, Yoshiko and 鬼頭 靖司 and 舘野 真太郎 and 太田 有紀},
 journal = {Journal of Mammalian Ova Research},
 month = {Apr},
 note = {In vitro fertilizability and subsequent developmental competence of inbred RFM/Ms mice, which have high incidence of myeloid leukemia by radiation, was studied.  When response of RFM/Ms females to various amount of eCG (1.25-10.0 i.u.) was examined, superovulation (>20 ovulated ova) occurred in animals injected with ³5.0 i.u. eCG.  The kinetics of in vitro fertilization and embryo development were examined and compared among RFM/Ms, C57BL/6J zygotes and their hybrid zygotes.  The fertilization kinetics was significantly slower in RFM/Ms zygotes than in C57BL/6J zygotes.  Crossbreeding experiments between C57BL/6J and RFM/Ms showed that the speed and incidence of fertilization were affected by paternal and maternal factors, respectively.  The in vitro development to the blastocyst stage of RFM/Ms zygotes was significantly slower than that of C57BL/6J zygotes and their hybrid zygotes at 96h post-insemination (PI), but not at 120h PI.  The nuclear number of RFM/Ms embryos was significantly lower than that of C57BL/6J and their hybrid embryos throughout the culture period (72, 96 and 120h PI).  Conversely, hatching at 120h PI in C57BL/6J embryos was much slower than that of the other groups of embryos.  This study showed that gametes and zygotes of RFM/Ms strain can be fertilized and developed well in vitro and that maternal and paternal factors and their interaction have complicated effects on fertilization and developmental kinetics in vitro.},
 pages = {32--38},
 title = {Kinetics of in vitro fertilization and development of an inbred mouse strain: study using RFM/Ms compared with C57BL/6J.},
 volume = {19},
 year = {2002}
}