| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-02 |
| タイトル |
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タイトル |
Competitive transplantation in rat mammary tissue and its use for cell competition and tumor clonality assays |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Nagata Kento
Nishimura-Yano Yukiko
Hosoki Ayaka
Kudo Ken-ichi
Daino Kazuhiro
Nishimura Mayumi
Imaoka Tatsuhiko
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Competitive transplantation is a valuable technique in experimental biology, although its use in mammary gland biology has been limited to mouse models. We aim to implement this technique in rats, an alternative model relevant to the human breast. Mammary cells were obtained from wild-type or transgenic rats expressing green or red fluorescent protein and transplanted into the cleared fat pads of recipients. Tissue clearing was implemented for the rat mammary gland to evaluate fluorescent proteins by confocal imaging. Transgenic rats were used as recipients because wild-type recipients rejected transgenic cells. A competitive assay between radiation-exposed and nonexposed cells expressing different fluorescence revealed no effect of high-dose radiation on the repopulating ability. A polyclonal nature was identified in adenomas developing in recipients that received a mixture of fluorescent and nonfluorescent cells and were exposed to carcinogens. Thus, we developed a method to track multiple cell lineages in the rat mammary gland. |
| 書誌情報 |
Journal of Radiation Research
発行日 2026-03
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1349-9157 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/jrr/rrag007 |
