| アイテムタイプ |
会議発表用資料 / Presentation(1) |
| 公開日 |
2025-10-27 |
| タイトル |
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|
タイトル |
P53-independent bystander effects through cell-to-cell communication between carbon-ion irradiated tumor and non-irradiated normal cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6670 |
|
資源タイプ |
conference poster |
| 著者 |
Suzuki Masao
Funayama Tomo
Suzuki Michiyo
Sai Sei
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| 抄録 |
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内容記述 |
Communication of signaling events from carbon-ion irradiated tumor to non-irradiated normal cells is one of major concerns for heavy-ion radiotherapy. Human lung adenocarcinoma cells (A549; wild-type P53) and human glioblastoma cells (T98G; mutated type P53) were irradiated with carbon-ion microbeams (12C5+, 220 MeV , LET=103keV/µm) produced with the Takasaki Ion Accelerator for Advanced Radiation Application (TIARA) in National Institutes for Quantum Science and Technology (QST), simulating the spot scanning irradiation system. Then non-irradiated normal human fibroblasts (NB1RGB; wild-type P53) were co-cultured with the irradiated tumor cells in presence or absence of a gap-junction inhibitor using the transwell permeable support system. Cell-killing effect and gene mutation at hypoxanthine guanine phosphoribosyltransferase (HPRT) locus in non-irradiated co-cultured NB1RGB cells in absence of the inhibitor were much higher than those of expected, assuming no bystander effects. However, no biological effects were induced, when using the presence of the inhibitor. The results suggested that the bystander cellular effects were induced in the non-irradiated normal cells being located close to direct irradiated tumor cells through gap-junction-mediated cell-to-cell communication and P53-gene status of irradiated tumor cells had no part in inducing the bystander effects. |
| 会議概要（会議名, 開催地, 会期, 主催者等） |
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内容記述 |
The 68th Japanese Radiation Research Society and The 6th Asian Congress of Radiation Research |
| 発表年月日 |
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日付 |
2025-10-25 |
