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Mechanical Insights into Nucleosome Remodeling by divalent Cations: A Single-molecule study
https://repo.qst.go.jp/records/2002135
https://repo.qst.go.jp/records/20021359b9d3846-8706-4427-b51e-1215e2cfbdff
| アイテムタイプ | 会議発表用資料 / Presentation(1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-10-24 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Mechanical Insights into Nucleosome Remodeling by divalent Cations: A Single-molecule study | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6670 | |||||||||||||||
| 資源タイプ | conference poster | |||||||||||||||
| 著者 |
Amarjeet Kumar
× Amarjeet Kumar
× Sunami Tomoko
× Sato Shoko
× Kurumizaka Hitoshi
× Kono Hidetoshi
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| 抄録 | ||||||||||||||||
| 内容記述 | Nucleosome remodeling dynamics regulate genome accessibility via unstacking and unwrapping. These highly dynamic processes are critical for transcription and replication. Using dual-trap optical tweezers, we studied 4-mer and 12-mer nucleosomal arrays (Widom-601 repeats) under divalent cations (Mg²⁺, Ca²⁺). Without Mg²⁺, outer and inner DNA turns unwrap at low (0–10 pN) and high (10–40 pN) forces, respectively, with rupture events matching nucleosome counts. Mg²⁺ lowers unwrapping forces and induces large disruptions, suggesting nucleosome unstacking. Rupture frequency stays unchanged, indicating Mg²⁺ enhances stacking while easing DNA removal. Overall, Mg²⁺ modulates chromatin mechanics, balancing accessibility, and compaction. | |||||||||||||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||||||||||||
| 内容記述 | The 63rd Annual Meeting of the Biophysical Society of Japan | |||||||||||||||
| 発表年月日 | ||||||||||||||||
| 日付 | 2025-09-24 | |||||||||||||||
