| アイテムタイプ |
会議発表用資料 / Presentation(1) |
| 公開日 |
2025-06-25 |
| タイトル |
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|
タイトル |
Biological Nitrate Remediation: Mutagenesis of Denitrifying Bacteria Using Ion Beam and Gamma Irradiation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6670 |
|
資源タイプ |
conference poster |
| 著者 |
Jan Nie Hing
Sato Katsuya
Shin Okazaki
|
| 抄録 |
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内容記述 |
The growing global population and food demand have led to increased fertilizer use, causing widespread nitrate contamination of water resources. Runoff from fertilizers and animal waste contaminates surface and groundwater, harming ecosystems and human health through algal blooms, eutrophication, and aquatic deaths. A sustainable solution for mitigating nitrate pollution involves the utilization of denitrifying bacteria for nitrate removal. This study aimed to create mutants of denitrifying bacteria using ion beam and gamma irradiation, and to explore the molecular mechanisms of denitrification in both wild-type and mutant strains. Five strains—Pseudomonas stutzeri JCM 5965, P. stutzeri TR2, Azospira sp. I09, Azospira sp. I13, and Bradyrhizobium ottawaense SGO9—were selected based on their well-documented genome sequences and ability to grow aerobically. Both ion beam and gamma radiation are forms of ionizing radiation, with ion beam irradiation being especially effective because of its high linear energy transfer. Irradiations conducted at QST Takasaki, Japan, revealed that ion beams were more lethal than gamma radiation. Enhanced strains will be applied to contaminated water for efficient nitrate removal. |
| 会議概要（会議名, 開催地, 会期, 主催者等） |
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|
内容記述 |
The growing global population and food demand have led to increased fertilizer use, causing widespread nitrate contamination of water resources. Runoff from fertilizers and animal waste contaminates surface and groundwater, harming ecosystems and human health through algal blooms, eutrophication, and aquatic deaths. A sustainable solution for mitigating nitrate pollution involves the utilization of denitrifying bacteria for nitrate removal. This study aimed to create mutants of denitrifying bacteria using ion beam and gamma irradiation, and to explore the molecular mechanisms of denitrification in both wild-type and mutant strains. Five strains—Pseudomonas stutzeri JCM 5965, P. stutzeri TR2, Azospira sp. I09, Azospira sp. I13, and Bradyrhizobium ottawaense SGO9—were selected based on their well-documented genome sequences and ability to grow aerobically. Both ion beam and gamma radiation are forms of ionizing radiation, with ion beam irradiation being especially effective because of its high linear energy transfer. Irradiations conducted at QST Takasaki, Japan, revealed that ion beams were more lethal than gamma radiation. Enhanced strains will be applied to contaminated water for efficient nitrate removal. |
| 発表年月日 |
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|
日付 |
2025-05-29 |
