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内容記述 |
Fucoxanthin is one of the light-harvesting pigments that is reported to have antioxidant, anticancer, and antidiabetic effects[1]. However, its biosynthetic pathway is not fully understood. In the present study, we aimed to isolate and analyze fucoxanthin-deficient mutants of the haptophyte Tisochrysis lutea with irradiation of heavy ion beams. The cells of T. lutea (C8-14 strain[2]) suspended in MA-ESM (artificial seawater) medium were irradiated with 45 to 120 Gy of carbon ion beams and then spread on agar-solidified MA-ESM. The survival rate decreased upon the irradiation dose increase, reaching approximately 10% at 65 Gy (Figure 1). We isolated two green-colored mutants, namely GR1 and GR2, after irradiation at 45 Gy and 100 Gy, respectively.Analysis of the pigments by liquid chromatography equipped with mass spectrometry (LC-MS) revealed that the green mutants lost fucoxanthin and implied that each GR1 and GR2 strain may accumulate haptoxanthin and phaneroxanthin, respectively, that are reported fucoxanthin precursors[3, 4]. We subsequently determined the whole-genome sequence in both strains and found that a gene for zeaxanthin epoxidase 1 (ZEP1) and a gene for carotenoid isomerase 5 (CRTISO5) were mutated in the GR1 and GR2, respectively. ZEP1 converts haptoxanthin into phaneroxanthin, and CRTISO5 converts phaneroxanthin into fucoxanthin. The similarities between the orthologs from the diatom Phaeodactylum tricornutum and T. lutea were 68% and 75%, respectively. This is the first findings of the genes for fucoxanthin synthesis in T. lutea. |
