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内容記述 |
Real-time monitoring of metabolism with various molecular probes is possible using the DNP (Dynamic Nuclear Polarization)-NMR (Nuclear Magnetic Resonance)/MRI (Magnetic Resonance Imaging) technique. It is especially important to generate various molecular probes of amino acids, which are biological components as templates, from the viewpoint of in vivo application. Therefore, DNP-NMR research using amino acids labeled with NMR-positive nuclei will expand the scope of future DNP-NMR applications. In this study, we have synthesized the tyrosine having 13C in the aromatic ring and optimized the conditions for hyperpolarization. Using the hyperpolarized tyrosine, the oxidative reaction catalyzed by tyrosinase was successfully monitored on a DNP-NMR spectra. This strategy might be applied to the detection of tyrosine metabolism in vivo. |
