| アイテムタイプ |
会議発表論文 / Conference Paper(1) |
| 公開日 |
2026-01-09 |
| タイトル |
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|
タイトル |
Fundamental examination of intracellular distribution of uranium in renal tubular cells that retain ureteral and vascular polarity |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
|
資源タイプ |
conference paper |
| アクセス権 |
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|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
Takeda Shino
Tanaka Izumi
Fujishiro Hitomi
Taguchi Hiroki
Terauchi Miyu
Yakumaru Haruko
Ayama Kyoko
Himeno Seiichiro
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The kidney is one of the key organs for the dose assessment after internal exposure to radionuclides because the internal radionuclides metabolized in the body are reabsorbed and excreted by the kidney and their renal retention is estimated to be high. The renal proximal tubules are responsible for the reabsorption of internal radionuclides from urine. The proximal tubules maintain polarity in vivo but the dynamics of the elemental distribution in the proximal tubules are not well understood. Uranium is an internal radionuclide and has a potential of nephrotoxicity. In the present study, using elemental imaging techniques by particle-induced X-ray emission analysis with microbeam (micro-PIXE) and synchrotron radiation X-ray fluorescence (SR-XRF) analyses, the intracellular distribution of uranium was examined in cell lines derived from the mouse proximal tubules that retain ureteral and vascular polarity.The cells were cultured with a trans-well culture system and the vertical cryo-sections were subjected for micro-PIXE and SR-XRF imaging. Clear elemental images corresponded to the cell arrangement were obtained for specimens 5 – 10 μm thick by micro-PIXE for sulfur, phosphorus, and potassium, and by SR-XRF for zinc and uranium. It was observed that the uranium was taken up and localized in the cells after exposure to uranium from the apical side. Quantitative evaluation for the localized uranium in cells by thin section standards will also be presented. |
| 書誌情報 |
The Journal of Toxicological Sciences
巻 50,
号 Suppl,
p. S159,
発行日 2025-07
|
