| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-01-08 |
| タイトル |
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タイトル |
myCRP, cysteine-rich protein in the yesso scallop Mizuhopecten, is a novel high-molecular weight Cd-binding protein |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Zheng Zehua
Namikawa Yuto
Awaji Masahiko
Kato Yugo
Iimura Kurin
Nakakido Makoto
Tsumoto Kouhei
Negishi Lumi
Kurumizawa Hitoshi
Takeda Shino
Suzuki MIchio
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Heavy metal bioaccumulation in marine organisms is a critical concern for the oceanic environment and food safety. Scallops, as filter-feeding bivalves, accumulate cadmium (Cd) in their midgut gland to toxic concentrations that may pose risks to human health. Despite extensive research on Cd bioaccumulation in the Yesso scallop Mizuhopecten yessoensis, a major Cd-binding protein has not yet been functionally characterized. In this study, we used high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) to isolate Cd-binding proteins, and identified myCRP, a novel high-molecular-weight Cd-binding protein. myCRP contains a cysteine-rich sequence, resembling metallothionein Cd-binding motifs, and is expressed exclusively in the midgut gland. Cd-binding assays demonstrated that myCRP binds Cd in a dose-dependent manner. Immunostaining revealed the presence of a small cluster of myCRP-containing cells in the connective tissue surrounding digestive tubules in the midgut gland. Synchrotron radiation X-ray fluorescence (SR-XRF) analyses showed the distribution of Cd throughout the entire midgut gland tissue. We conclude that myCRP is partly responsible for Cd binding in the midgut gland. Our findings clarify a new mechanism of Cd-binding in marine organisms from both biological and molecular perspectives. |
| 書誌情報 |
Marine Biotechnology
巻 28,
p. 5,
発行日 2025-12
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| 出版者 |
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出版者 |
Springer |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/s10126-025-10553-7 |
