| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-03 |
| タイトル |
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タイトル |
Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow-twitch muscles |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Hamaguchi Hiroki
Oyama Tomoko
Oyama Kotaro
Yasuko Manabe
Nobuharu L. Fujii
Taguchi Mitsumasa
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Skeletal muscles are classified into slow-twitch muscles composed primarily of type I and IIa fibers with high oxidative metabolism, and fast-twitch muscles composed of type IIx and IIb fibers with high glycolytic metabolism. The shift of fiber types occurs during development and later in life, however, the stimuli that shift these types remain unclear. We analyzed the role of mechanical stimuli in myotube formation and shift to the characteristics of each fiber type using crosslinked gelatin gels with tunable elasticity (10–230 kPa) and microgrooves (3–50 µm). C2C12 myotubes on 10 kPa gel increased the expression of marker genes for type I and IIa fibers (MYH7 and MYH2) and oxidative metabolism (GLUT4 and myoglobin) than those on stiffer gels. Upregulation of PGC-1α on soft gel indicated to induce a shift of myotubes to the genetic characteristics of slow-twitch muscles. Microgrooves (3–10 µm) enhanced myoblast fusion and myotube orientation, without affecting the gene expressions characterizing fiber types. This study demonstrated an approach to create highly oriented slow-twitch muscle models by controlling the elasticity and microgrooves. |
| 書誌情報 |
Scientific Reports
巻 15,
号 27825,
発行日 2025-08
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| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
40781258 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41598-025-12744-7 |
