| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-10 |
| タイトル |
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タイトル |
Type I collagen gels for assessing the combined effects of ligand concentration-dependent elasticity and fibril density on cells |
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言語 |
ja |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
大山 智子
大山 廣太郎
三好 洋美
田口 光正
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Type I collagen (Col-I) is the most abundant fibrous protein in the extracellular matrix (ECM) and its densification is involved in the progression of various diseases and aging. Elevated Col-I concentrations increase ECM fibril density and stiffening; therefore, the effects of such changes in the microenvironment of cells require examination. To separately examine the effect of ligand concentration-dependent elasticity (C-E) and fibril density of Col-I, we developed three types of Col-I gels by introducing reagent-free radiation-induced crosslinking while adjusting the distance between the Col-I monomers or fibrils. The adhesion area of HeLa human cervical cancer epithelial cells, human induced pluripotent stem (hiPS) cells, and 3T3-Swiss albino mouse embryonic fibroblasts, tended to decrease with increasing fibril density when C-E was constant. In the absence of fibrils, the adhesion area of HeLa and 3T3-Swiss cells increased with increasing C-E, whereas hiPS cells clustered regardless of C-E. When C-E and the fibril density increased simultaneously, the adhesion area of HeLa and 3T3-Swiss cells remained unchanged due to the two opposing effects. Our results highlight the importance of evaluating the combined compositional, mechanical, and topographical effects of Col-I on cells. The developed gels will contribute to elucidating the role of Col-I in biological functions. |
| 書誌情報 |
Materials & Design
巻 258,
p. 114608,
発行日 2025-10
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| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.matdes.2025.114608 |
