| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-01 |
| タイトル |
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タイトル |
Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics. |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Min Fey Chek
Sun-Yong Kim
Tomoyuki Mori
Keiji Matsumoto
Shunsuke Sato
Toshio Hakoshima
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Polyhydroxyalkanoate (PHA) is a biodegradable polyester that can serve as a promising alternative to petrochemical plastics which present a serious source of pollution. PHA synthase (PhaC) is a key enzyme responsible for producing a wide variety of PHAs in microorganisms. Here we present crystal structures of full-length PhaC from?Aeromonas caviae, a high-performance PhaC employed for industrial use. The structure reveals an N-terminal helical domain that mediates head-to-head dimerization and stabilizes the C-terminal α/β catalytic domain to form a tunnel that connects the catalytic center embedded inside the protein to the protein surface. We showed that this tunnel is a putative egress tunnel for the product PHA chain. Our results establish a fundamental understanding of the PhaC machinery that should lead to improvement of this enzyme in industrial applications. |
| 書誌情報 |
Angewandte Chemie (International ed. in English)
p. e202504626,
発行日 2025-01
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| 出版者 |
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出版者 |
Wiley-VCH |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1521-3773 |
| PubMed番号 |
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|
識別子タイプ |
PMID |
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関連識別子 |
40276819 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/anie.202504626 |
