| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-04-26 |
| タイトル |
|
|
タイトル |
Microbeam irradiation reveals cytoplasmic damage contributes to micronuclei induction. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Konishi Teruaki
Wang Jun
Kobayashi Alisa
Cuihua Liu
Hiroyama Yota
Mamiya Taisei
Kusumoto Tamon
Oikawa Masakazu
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Microbeam technology offers a valuable tool to investigate inter- and intracellular responses to radiation-induced damage. While direct nucleus irradiation is known to be the primary cause of various radiobiological effects, the cytoplasm also receives radiation and potentially influencing intracellular responses. This study aims to determine whether cytoplasmic irradiation contributes to micronuclei induction when the cytoplasm and nucleus are sequentially irradiated.
Microbeam irradiation were performed using the SPICE-QST microbeam at the National Institutes for Quantum Science and Technology, delivering 3.4 MeV protons (LET in water: 11.7 keV/μm) with a 2 μm beam diameter. Three targeted irradiations were employed: (N) nucleus-only, (C) cytoplasm-only, and (N+C) irradiating both the nucleus and cytoplasm. In (N+C), with 1000 protons delivered to the cytoplasm in the N+C group. Immediately post-irradiation, the cyto-block method was employed for micronucleus (MN) formation analysis. Cells were fixed 50 hours later, followed by staining the cytoplasm and nucleus with Cell Tracker Orange (10 μg/ml) and Hoechst33342 (1 μg/ml), respectively. Fluorescence images of over 1000 binucleated cells were captured using the SPICE offline microscopic system.
The MN induction rate per bi-nucleated cell was compared between N and N+C (1000 protons) irradiation. N irradiation displayed a peak MN induction rate at around 100 protons, whereas N+C irradiation peaked at around 300 protons. The result implies that the cytoplasmic irradiation induced protective response against MN induction. Notably, at 1000 protons or more, N+C irradiation yielded an MN induction rate approximately twice that of N irradiation. This may be result of enhanced cellular progression triggered by cytoplasmic damage. These findings clearly demonstrate that not only DNA damage but also cytoplasmic damage contribute significantly to MN induction, suggesting a more complex interplay between cellular organelles during radiation exposure. |
| 書誌情報 |
The European Physical Journal Plus
巻 140,
p. 334,
発行日 2025-04
|
| 出版者 |
|
|
出版者 |
EDP Sciences, Societa Italiana di Fisica and Springer Berlin Heidelberg |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1140/epjp/s13360-025-06265-5 |
