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Intracultivar DNA identification in Cymbidium using DNA polymorphisms generated using simple genome scanning with random primers and heavy-ion beam irradiation
https://repo.qst.go.jp/records/2001310
https://repo.qst.go.jp/records/200131067d96ea8-fdba-4c81-9ad8-0f04ed087a57
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||||
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| 公開日 | 2024-09-09 | |||||||||||||||||||||||||||
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| タイトル | Intracultivar DNA identification in Cymbidium using DNA polymorphisms generated using simple genome scanning with random primers and heavy-ion beam irradiation | |||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||
| 著者 |
Koji Furukawa
× Koji Furukawa
× Yoko Ono
× Shimokawa Takashi
× Kitamura Hisashi
× Tomoko Abe
× Toshikazu Ebisuzaki
× Norihito Saito
× Satoshi Wada
× Shusei Sato
× Satoshi Tabata
× Matsuyama Tomoki
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| 抄録 | ||||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||
| 内容記述 | DNA marker-based discrimination between a wild-type specimen and mutants obtained using heavy-ion beams (because of small genomic and genetic mutations) is challenging in mutation breeding. Protecting the plant breeders’ right to mutate cultivars is challenging because tissue culture techniques that use the protocorm-like body (PLB) can quickly propagate. Thus, we present two methods for generating DNA markers in Cymbidium using genomic mutations induced by heavy-ion beams. In the first method, we selected 8?16 plants from three cultivars after irradiation that had no mutations in morphology or traits, followed by genome scanning using 240 sets of 15-mer random amplified polymorphic DNA primers and arbitrarily primed PCR primers based on partial retrotransposon sequences. Three polymorphic patterns were generated in each cultivar. In the second method, cultivar candidates were generated by crossbreeding, and a strain was selected. Genome scanning was performed on 43 plants irradiated by applying a carbon- or neon-ion beam to the PLB and using five random primer sets that allowed many scorable bands. Polymorphic patterns were detected in two strains at all micropropagation steps via the PLB and in second-flowering plants. These data demonstrate that strains with the same appearance after heavy-ion beam irradiation can be distinguished using polymorphic DNA patterns alone. These patterns were defined as “DNA marks” for intracultivar identification. In cases where multiple strains of DNA marks are obtained in one cultivar, DNA marks can realize the feasibility of DNA-level traceability from production to market. |
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| 書誌情報 |
Scientia Horticulturae 発行日 2024-11 |
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