| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-03-08 |
| タイトル |
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タイトル |
Mutation detection of urinary cell-free DNA via catch-and-release isolation on nanowires for liquid biopsy. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Hiromi Takahashi
Takao Yasui
Masaki Hirano
Keiko Shinjo
Yusuke Miyazaki
Wataru Shinoda
Takeshi Hasegawa
Atsushi Natsume
Yotaro Kitano
Mikiko Ida
Min Zhang
Taisuke Shimada
Piyawan Paisrisarn
Zetao Zhu
Fumiharu Ohka
Kosuke Aoki
Sakon Rahong
Kazuki Nagashima
Takeshi Yanagida
Baba Yoshinobu
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Cell-free DNA (cfDNA) and extracellular vesicles (EVs) are molecular biomarkers in liquid biopsies that can be applied for cancer detection, which are known to carry information on the necessary conditions for oncogenesis and cancer cell-specific activities after oncogenesis, respectively. Analyses for both cfDNA and EVs from the same body fluid can provide insights into screening and identifying the molecular subtypes of cancer; however, a major bottleneck is the lack of efficient and standardized techniques for the isolation of cfDNA and EVs from clinical specimens. Here, we achieved catch-and-release isolation by hydrogen bond-mediated binding of cfDNA in urine to zinc oxide (ZnO) nanowires, which also capture EVs by surface charge, and subsequently we identified genetic mutations in urinary cfDNA. The binding strength of hydrogen bonds between single-crystal ZnO nanowires and DNA was found to be equal to or larger than that of conventional hydrophobic interactions, suggesting the possibility of isolating trace amounts of cfDNA. Our results demonstrated that nanowire-based cancer screening assay can screen cancer and can identify the molecular subtypes of cancer in urine from brain tumor patients through EV analysis and cfDNA mutation analysis. We anticipate our method to be a starting point for more sophisticated diagnostic models of cancer screening and identification. |
| 書誌情報 |
Biosensors & bioelectronics
巻 234,
p. 115318,
発行日 2023-08
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| 出版者 |
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出版者 |
Elsevier Advanced Technology |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1873-4235 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
37172361 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.bios.2023.115318 |
