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Fibrillization Process of Human Amyloid-Beta Protein (1?40) under a Molecular Crowding Environment Mimicking the Interior of Living Cells Using Cell Debris
https://repo.qst.go.jp/records/2000787
https://repo.qst.go.jp/records/200078795684a41-2a45-4844-bc28-f4248c9e3445
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2024-12-20 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Fibrillization Process of Human Amyloid-Beta Protein (1?40) under a Molecular Crowding Environment Mimicking the Interior of Living Cells Using Cell Debris | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Mitsuhiro Hirai
× Mitsuhiro Hirai
× Arai Shigeki
× Hiroki Iwase
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | We have demonstrated a novel scattering method capable of selectively detecting the structures of targeted proteins or membranes in a closely mimicking intracellular milieu achieved utilizing whole-cell contents. This method relies on the inverse contrast matching technique in neutron scattering. By employing this method, we successfully observed the fibrillization process of human amyloid beta-protein (Aβ 1?40) under a molecular crowding environment (13.1% w/v cell debris, Aβ/cell debris = ~1/25 w/w) that closely mimics the interior of living cells. Aβ protein is well known as a major pathogenic component of Alzheimer’s disease. The present results combining model simulation analyses clearly show that the intracellular environment facilitates the potential formation of even more intricate higher-order aggregates of Aβ proteins than those previously reported. | |||||||||||
| 書誌情報 |
Molecules 巻 28, 号 18, p. 6555, 発行日 2023-09 |
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| 出版者 | MPDI | |||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1420-3049 | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.3390/molecules28186555 | |||||||||||
