| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-10-20 |
| タイトル |
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|
タイトル |
Simultaneous production of cellobiose and xylobiose from alkali-treated bagasse using cellulase secreted by Fe-ion-irradiated Trichoderma reesei mutant |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Noguchi Takuya
Ryuji Nishiyama
Shimokawa Takashi
Katsushige Yamada
Yusuke Kagawa
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Cellobiose and xylobiose are disaccharides composed of two glucose or xylose units with β-1,4 linkages. This study aimed to isolate a Trichoderma reesei mutant that lacks β-glucosidase and β-xylosidase activities for the simultaneous production of these disaccharides. Mutagenesis using Fe-ion beam resulted in a mutant strain, T. reesei T1640; the cellulase production in this strain was as high as that in the parent strain. Genomic analysis revealed that T1640 lost both the β-glucosidase and β-xylosidase activities owing to the translocation of the responsible genes. Hydrolysis of alkali-treated bagasse using the enzymes from T1640 leads to high yields (365 mg/g-biomass) and ratios (72.7% of the total sugars) of cellobiose and xylobiose. |
| 書誌情報 |
Journal of Bioscience and Bioengineering
巻 134,
号 6,
p. 491-495,
発行日 2022-12
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1389-1723 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
36220721 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.jbiosc.2022.09.002 |
