量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Objective: SPECT/PET/CT is useful to analyze behaviors of two probes with different functions. Recently, a new concept of SPECT/PET/CT imaging with VECTor small-animal scanner (MILabs) has been developed, which allows to obtain truly simultaneous dual-isotope images for SPECT/PET nuclides with high resolution by separating the photon energy using the collimator with clustered pinholes (Goorden et al. JNM 2013). In this study, we evaluated the quantitativity of simultaneous measurement with SPECT nuclide 99mTc and PET nuclide 64Cu using this system by phantom study and explored its in vivo application. Methods: Phantoms containing single isotope (99mTc or 64Cu) or dual isotopes with various concentrations and ratios were used. Radioactivity of the phantoms was measured by SPECT/PET/CT system. In in vivo study, simultaneous imaging with a hypoxia-detecting agent 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) and a blood pool agent 99mTc-human serum albumin (99mTc-HSA), was performed using HT-29 tumor-bearing mice. Results: In phantom study, radioactivity measured by SPECT/PET imaging was consistent with that enclosed in the phantoms, which was similar in both single- and dual-isotope phantoms (99mTc: 0-9.25 MBq, 64Cu: 0-18.5 MBq). The results were same, even when ratios were changed. In vivo study revealed that the distribution of hypoxic regions detected by 64Cu-ATSM was clearly distinguished from blood pool detected by 99mTc-HSA within HT-29 tumors. Conclusions: We showed the quantitativity of the SPECT/PET/CT system with 99mTc and 64Cu from phantom study. Also, we demonstrated the capability of the system in in vivo imaging with 64Cu-ATSM and 99mTc-HSA to simultaneously analyze tumor hypoxia and vascularity.
会議概要（会議名, 開催地, 会期, 主催者等）
The International Chemical Congress of Pacific Basin Socie 2015
Evaluation of simultaneous dual-isotope SPECT/PET/CT imaging with 99mTc and 64Cu by phantom study and its in vivo application
