量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Exposure to either ionizing radiation (IR) or psychological stress (PS) could cause detrimental effects on humans. A recent study showed that chronic restraint induced PS (CRIPS) could attenuate Trp53 functions and increase radiocarcinogenesis in Trp53-heterozygous mice, having a big impact on the academic and a sensational effect on the public, especially the residents living in the radioactively contaminated areas. To elucidate possible modification effects from CRIPS on radiation-induced health consequences in Trp53 wild type (Trp53wt) animals, a series of investigation with multidisciplinary analyses was done. We demonstrate here the changes in frequency of chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53wt C57BL/6J mice were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) at a dose of 4 Gy was given on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with the fluorescence in situ hybridization (FISH) probes for chromosomes 1 (green), 2 (red) and 3 (yellow). Results showed that CRIPS alone did not induce CAs, while TBI
caused significant increase of CAs, mostly stable types (translocations and insertions). Intriguingly, the stable-type CAs appeared at a little high frequency in mice exposed to TBI plus CRIPS than those in mice exposed to TBI alone. Whereas, the frequency of unstable-type CAs (dicentrics and fragments) was not differ between these experimental groups (TBI+CRIPS vs. TBI). CRIPS does not seem to have a significant impact on initiation of radiocarcinogenesis of splenocytes because clastogenic effect of TBI was not enhanced by CRIPS. Further investigations elucidating possible modification effects from CRIPS on promotion and/or progression of radiocarcinogenesis of Trp53wt mice must be needed.
Effects from chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes
