量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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PURPOSE: To investigate whether nitric oxide excreted from irradiated cells with accelerated carbon ion beams modulates cellular radiosensitivity against the accelerated carbon ion beams in human glioblastoma cells bearing either wild-type p53 or mutant p53.
MATERIALS AND METHODS: Western blot analysis of inducible nitric oxide synthase, hsp72 and p53, the concentration assay of nitrite in medium and cell survival assay after irradiation with accelerated carbon ion beams were performed.
RESULTS: The accumulation of inducible nitric oxide synthase was caused by accelerated carbon ion beam irradiation of mutant p53 cells but not of wild-type p53 cells. The accumulation of hsp70 and p53 was observed in wild-type p53 cells after exposure to the conditioned medium from the irradiated mutant p53 cells with accelerated carbon ion beams, and the accumulation was abolished by the addition of an inhibitor for inducible nitric oxide synthase to the medium. The radiosensitivity of wild-type p53 cells was reduced in the conditioned medium from the irradiated mutant p53 cells with accelerated carbon ion beams compared with conventional fresh growth medium, and the reduction of radiosensitivity was abolished by the addition of a specific inducible nitric oxide synthase inhibitor to the conditioned medium.
CONCLUSIONS: Nitric oxide excreted from the irradiated donor cells with accelerated carbon ion beams could modulate radiosensitivity of recipient cells against the accelerated carbon ions. These findings indicate the importance of an intercellular signal transduction pathway initiated by nitric oxide in the cellular response to accelerated heavy ions.
会議概要（会議名, 開催地, 会期, 主催者等）
The 3rd National Meeting of Ion Beam Bioengneering and The 1st International Symposium on Ion Beam: Biological Effects and Molecular Mechanisms
Nitric oxide is an initiator of the bystander effect induced by heavy-ion beams
